System Biology

whole > sum of its part

What system biology not... NOT a black office, NOT a Spherical Cow',

A matter of sacle- physics, chemistry , biology

A Fundamental Difference-- physics, chemistry= predictive, but, biology= observational

Making biology a predictive science

why do we need computational modelling?

A new way of drug design- system focus<- target focus<- legend focus Reconstruction of Metabolic Networks Practice of systems biology Stoichiometric Analysis- capture the structure of metabolic network, does not require quntitative kinetic parameters, FBA (Flux balance analysis) Work Flow of FBA -- Development of pathway model-> computation of stoichiometric matrix-> FBA-->in silico gene deletions-> drug perturbations-> identification of protein essential to pathway-> Drug target

Mycolic Acid Biosynthesis Pathway

Reactome Modelling -- Pathway Modelling (FBA), Network Modelling

Reaction Networks: Comparative Analysis

targetTB: Target Identification Pipeline

Network Analysis- Node deletions: change in netwok parameters; Lethal Deletions

Interactome Construction

Structure Analysis

PocketDepth: Binding pocket detection

PocketMatch- A new algorithm for Binding site comparison

Protein-Protein Influence Matrix: identify maximally connected code; Preliminary analysis shows hub involvement.

High Order Interactions: Pathway-Pathway; Process-Process

Pathways to Drug Resistant

Model Summary: model construction from litreature, Boolean construction, 75 nodes are present.

State MAP for simulations

MAP of single & double knock-outs

work flow- host pathogen modelling: advantage of modelling- bird's eye view for host-pathogen interplay

Genomics-> Proteomics-> System Biology